Primer

Part:BBa_K3568004:Design

Designed by: MENG XU   Group: iGEM20_XH-China   (2020-10-21)


pTrcHis Reverse Primer


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This reverse primer design to satisfied the sequencing need and PCR. So it should be locate downstream of multiple cloning site and stop codon.


Source

This primer has designed according to the pTrcHis plasmid sequence.

References